ABSTRACT
The ever-increasing demand for wildlife-derived raw or processed meat commonly known as bushmeat, has been identified as one of the critical factors driving the emergence of infectious diseases. This study focused on examining the bacterial community composition of smoked and fermented bushmeats, specifically grasscutter, rat, rabbit, and mona monkey. The analysis involved exploring 16Sr RNA amplicon sequences isolated from bushmeat using QIIME2. Microbiome profiles and their correlation with proximate components (PLS regression) were computed in STAMP and XLSTAT, respectively. Results indicate the predominance of Firmicutes (70.9%), Actinobacteria (18.58%), and Proteobacteria (9.12%) in bushmeat samples at the phylum level. Staphylococcus, Arthrobacter, Macrococcus, and Proteus constituted the core microbiomes in bushmeat samples, ranked in descending order. Notably, significant differences were observed between the bacterial communities of bushmeat obtained from omnivores and herbivores (rat and mona monkey, and grasscutter and mona monkey), as well as those with similar feeding habits (rat and monkey, and grasscutter and rabbit) at the family and genus levels. Each type of bushmeat possessed unique microbial diversity, with some proximate components such as fat in rat samples correlating with Staphylococcus, while proteins in Mona monkey correlated with Arthrobacter and Brevibacterium, respectively. The study underscores public health concerns and highlights probiotic benefits, as bushmeat samples contained both pathogenic and beneficial bacteria. Therefore, future research efforts could focus on improving bushmeat quality.
ABSTRACT
Cocoa is one of the agricultural commodities which is highly susceptible to mycotoxin contamination. During two crop/harvest seasons, the occurrence and distribution of ochratoxin A (OTA) in viable commercial cocoa beans were investigated. The cocoa bean samples were collected randomly from farmers across cocoa-growing regions of Ghana. OTA concentrations in the samples were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods following purification on immunoaffinity solid phase column. The result showed that 21.7% of all samples analyzed were contaminated with OTA at concentrations ranging from 0.01 µg/kg to 12.36 µg/kg. The Western South region had the highest occurrence of OTA-positive samples at 32.5%, followed by the Western North region at 28.75%, the Eastern and Volta regions at 25% each, Brong Ahafo (16.25%), Central (15%) and the Ashanti region at 11.25%. However, 0.9% and 3.5% of the total OTA-positive samples exceeded the OTA maximum limits of 10 µg/kg for cocoa beans and 3 µg/kg for cocoa powder, set by the Brazilian National Health Surveillance Agency and the European Commission, respectively. During the Main and Light crop seasons, the highest concentrations of OTA were detected in the Western North region, reaching up to 12.36 µg/kg and 3.45 µg/kg, respectively. OTA concentrations between the cocoa-growing regions in the Main crop season were not significantly different (p > 0.05), however, the Light crop season indicated a significant difference (p < 0.05). There was a significant difference (p < 0.05) between the two crop seasons. The need for regular monitoring and careful adherence to agronomic strategies such as good agricultural practices (GAPs), recommended code of practices (COPs) and good manufacturing practices (GMPs) for the prevention and reduction of OTA throughout the cocoa value chain cannot be overemphasized.
ABSTRACT
There has been a concern of metal contamination from the mining areas which could expose the population to toxic metal through consumption of food products in the regions. Therefore, the study was conducted to analyze 18 elements using X-ray fluorescence analyzer and to assess for metal exposure through dietary intakes. Dietary recall survey instrument was used to collect consumption, demographic, and anthropometric data from 308 consumers in life-stage groups: toddlers, adolescents, and adults. Cassava, maize, rice, tomato, and yam indigenous diet samples (66) from the gold mining regions of Ghana were obtained by purposively sampling from street vendors. Principal component analysis was used to apportion pollution source. Health risks due to dietary elements were quantified using US EPA probabilistic protocols for cancer and non-cancer disease endpoints. Deficiency and overage risks of essential elements were tested against reference nutrient intakes. The element-diet-location mode of principal component analysis separated cassava and maize diets as significant vehicles for both essential and toxic elements apportioned to mining pollution source. The chronic daily intake trend revealed a preponderance for arsenic (maize: toddlers 0.009, adolescents 0.025 adults 0.010 mg/kg-d) and lead (maize: toddlers 0.009, adolescents 0.026, adults 0.010 mg/kg-d) exposure through maize diets compared to cassava diets. The hazard quotient showed (HQ > 1) life-stage risk differentiation for barium, although there were cumulative and pervasive health risks for all age groups based on the hazard index (Ag, Ba, Cd, Co, Hg, Mn, Mo, Sb, Sn, Sr, U, V, W, and Zr). The likely cancer risk (mode) for all life-stage groups (3 × 10-6-1 × 10-2) were also high in that they exceeded the 1 × 10-6 safety threshold. The modal margin of exposure (MoE < 1) alerted high public health concern due to lead-induced toxicities among all life-stage groups through all diet vehicles. Regarding nutritional adequacy, there were possible overage risks associated with selenium, including deficiency risks for calcium, potassium, and zinc for all life-stage groups. Toddlers and adolescents were susceptible to iron deficiency risks through cassava diets and iron overage risks through maize diets.
